|
KMID : 1101420190510040414
|
|
Korean Journal of Clinical Laboratory Science
2019 Volume.51 No. 4 p.414 ~ p.419
|
|
|
Diagnostic Method for the Detection of JC Polyomavirus Using Loop-mediated Isothermal Amplification
|
|
Cho Kyu-Bong
|
|
|
Abstract
|
|
|
|
JC polyomavirus (JCPyV) is a human pathogenic virus belonging to the family Polyomaviridae, a viral group containing dsDNA nucleic acid. A recent recommendation is to apply the presence of JCPyV as a fecal indicator for water contamination in environments like sewage, and techniques to monitor JCPyV in water are being proposed. To date, the conventional PCR system has been applied as a diagnostic method for detecting JCPyV. There is a need for a more rapid and sensitive JCPyV diagnostic detection method in clinical and environmental samples. In this study, we developed a loop-mediated isothermal amplification (LAMP) primer set for the detection of JCPyV. Our results indicate that the LAMP method using a specific primer set shows about 10-fold higher detection sensitivity than the conventional PCR system. The effectiveness of the LAMP method developed in this study has been validated by PCR product digestion using the HaeIII restriction enzyme. We, therefore, propose that the LAMP method using a specific primer set can be applied as a rapid and sensitive detection method for monitoring JCPyV in clinical and environmental samples.
|
|
|
KEYWORD
|
|
|
Fecal indicator, JCPyV, Loop-mediated isothermal amplification, Validation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|